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ProductsBack/GRASP1 antibody [N1N2], N-term
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: SK-N-SH 7.5% SDS PAGE GTX122295 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: SK-N-SH 7.5% SDS PAGE GTX122295 diluted at 1:1000
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: SK-N-SH 7.5% SDS PAGE GTX122295 diluted at 1:1000

GRASP1 antibody [N1N2], N-term

GTX122295
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetGRIPAP1
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Overview

  • Supplier
    GeneTex
  • Product Name
    GRASP1 antibody [N1N2], N-term
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID56850
  • Target name
    GRIPAP1
  • Target description
    GRIP1 associated protein 1
  • Target synonyms
    GRASP-1, GRIP1-associated protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ4V328
  • Protein Name
    GRIP1-associated protein 1
  • Scientific Description
    GRASP1 is a neuron-specific guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF) and is associated with the GRIP/AMPA receptor complex in brain (Ye et al., 2000 [PubMed 10896157]).[supplied by OMIM]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRASP1/GRIPAP1 using anti-GRASP1/GRIPAP1 antibody (A13005-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: huamn HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat panceras tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse pancreas tissue lysates, Lane 13: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRASP1/GRIPAP1 antigen affinity purified polyclonal antibody (Catalog # A13005-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRASP1/GRIPAP1 at approximately 96 kDa. The expected band size for GRASP1/GRIPAP1 is at 96 kDa.
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