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ProductsBack/GRASP1 (GRIPAP1) Mouse Monoclonal Antibody [Clone ID: OTI1E3]
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GRIPAP1 (Cat# RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIPAP1(Cat# TA500876). Positive lysates [LY402754] (100ug) and [LC402754] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GRIPAP1 (Cat# RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIPAP1(Cat# TA500876). Positive lysates [LY402754] (100ug) and [LC402754] (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY GRIPAP1 (Cat# RC210936, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRIPAP1(Cat# TA500876). Positive lysates [LY402754] (100ug) and [LC402754] (20ug) can be purchased separately from OriGene.

GRASP1 (GRIPAP1) Mouse Monoclonal Antibody [Clone ID: OTI1E3]

CF500876
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGRIPAP1
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    GRASP1 (GRIPAP1) Mouse Monoclonal Antibody [Clone ID: OTI1E3]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI1E3
  • Gene ID56850
  • Target name
    GRIPAP1
  • Target description
    GRIP1 associated protein 1
  • Target synonyms
    GRASP-1, GRIP1-associated protein 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ4V328
  • Protein Name
    GRIP1-associated protein 1
  • Scientific Description
    Carrier-free (BSA/glycerol-free) GRIPAP1 mouse monoclonal antibody, clone OTI1E3 (formerly 1E3)
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRASP1/GRIPAP1 using anti-GRASP1/GRIPAP1 antibody (A13005-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: huamn HepG2 whole cell lysates, Lane 3: human RT4 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human SiHa whole cell lysates, Lane 6: human T-47D whole cell lysates, Lane 7: rat brain tissue lysates, Lane 8: rat panceras tissue lysates, Lane 9: rat testis tissue lysates, Lane 10: rat PC-12 whole cell lysates, Lane 11: mouse brain tissue lysates, Lane 12: mouse pancreas tissue lysates, Lane 13: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRASP1/GRIPAP1 antigen affinity purified polyclonal antibody (Catalog # A13005-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRASP1/GRIPAP1 at approximately 96 kDa. The expected band size for GRASP1/GRIPAP1 is at 96 kDa.
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TargetGRIPAP1
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TargetGRIPAP1
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Sample (30 ug of whole cell lysate) A: HepG2 7.5% SDS PAGE GTX108063 diluted at 1:500
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TargetGRIPAP1
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