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ProductsBack/GRB2 (1-68): m-IgGkappa BP-HRP Bundle

GRB2 (1-68): m-IgGkappa BP-HRP Bundle

SC-533691
Santa Cruz Biotechnology
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot
Product group Antibodies
TargetGRB2
1 kit
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Overview

  • Supplier
    Santa Cruz Biotechnology
  • Product Name
    GRB2 (1-68): m-IgGkappa BP-HRP Bundle
  • Delivery Days Customer
    5
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID2885
  • Target name
    GRB2
  • Target description
    growth factor receptor bound protein 2
  • Target synonyms
    ASH, EGFRBP-GRB2, Grb3-3, MST084, MSTP084, NCKAP2, growth factor receptor-bound protein 2, HT027, SH2/SH3 adapter GRB2, abundant SRC homology, epidermal growth factor receptor-binding protein GRB2, epididymis secretory sperm binding protein, growth factor receptor-bound protein 3, protein Ash
  • Host
    Mouse
  • Isotype
    IgG2b
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRB2 using anti-GRB2 antibody (A00351-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB2 antigen affinity purified polyclonal antibody (Catalog # A00351-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB2 at approximately 25 kDa. The expected band size for GRB2 is at 25 kDa.
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