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Western blot All lanes: Growth factor receptor-bound protein 7 antibody at 2ug/ml + Rat brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 50, 63, 56 kDa Observed band size: 60 kDa
Western blot All lanes: Growth factor receptor-bound protein 7 antibody at 2ug/ml + Rat brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 50, 63, 56 kDa Observed band size: 60 kDa
Western blot All lanes: Growth factor receptor-bound protein 7 antibody at 2ug/ml + Rat brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 60, 50, 63, 56 kDa Observed band size: 60 kDa

GRB7 Antibody

CSB-PA009890ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetGRB7
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRB7 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2886
  • Target name
    GRB7
  • Target description
    growth factor receptor bound protein 7
  • Target synonyms
    B47; epidermal growth factor receptor GRB-7; GRB7 adapter protein; growth factor receptor-bound protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14451
  • Protein Name
    Growth factor receptor-bound protein 7
  • Scientific Description
    Adapter protein that interacts with the cytoplasmic domain of numerous receptor kinases and modulates down-stream signaling. Promotes activation of down-stream protein kinases, including STAT3, AKT1, MAPK1 and/or MAPK3. Promotes activation of HRAS. Plays a role in signal transduction in response to EGF. Plays a role in the regulation of cell proliferation and cell migration. Plays a role in the assembly and stability of RNA stress granules. Binds to the 5UTR of target mRNA molecules and represses translation of target mRNA species, when not phosphorylated. Phosphorylation impairs RNA binding and promotes stress granule disassembly during recovery after cellular stress (By similarity).
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRB7 using anti-GRB7 antibody (A02528). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB7 antigen affinity purified polyclonal antibody (Catalog # A02528) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB7 at approximately 60 kDa. The expected band size for GRB7 is at 60 kDa.
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