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ProductsBack/Growth factor receptor bound protein 7 Recombinant Antbody Kit (KD-Validated)

Growth factor receptor bound protein 7 Recombinant Antbody Kit (KD-Validated)

BSM-90375R
Bioss Antibodies
ApplicationsFlow Cytometry, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetGRB7
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    Growth factor receptor bound protein 7 Recombinant Antbody Kit (KD-Validated)
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, Western Blot, ImmunoCytoChemistry
  • Applications Supplier
    WB(1:300-5000), FCM(1:20-100), IC(1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID2886
  • Target name
    GRB7
  • Target description
    growth factor receptor bound protein 7
  • Target synonyms
    B47; epidermal growth factor receptor GRB-7; GRB7 adapter protein; growth factor receptor-bound protein 7
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14451
  • Protein Name
    Growth factor receptor-bound protein 7
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRB7 using anti-GRB7 antibody (A02528). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A431 whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRB7 antigen affinity purified polyclonal antibody (Catalog # A02528) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRB7 at approximately 60 kDa. The expected band size for GRB7 is at 60 kDa.
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