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GRHL2 Antibody (Biotin)

LS-C399428
LifeSpan BioSciences
ApplicationsELISA
Product group Antibodies
TargetGRHL2
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GRHL2 Antibody (Biotin)
  • Delivery Days Customer
    23
  • Application Supplier Note
    The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Applications
    ELISA
  • Applications Supplier
    ELISA The applications listed above are for the unconjugated form of this antibody. The conjugated antibody has not been tested. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Biotin
  • Estimated Purity
    ...
  • Gene ID79977
  • Target name
    GRHL2
  • Target description
    grainyhead like transcription factor 2
  • Target synonyms
    BOM, DFNA28, ECTDS, PPCD4, TFCP2L3, grainyhead-like protein 2 homolog, brother of mammalian grainyhead, brother-of-MGR, grainyhead-like 2, transcription factor CP2-like 3
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C,-80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GRHL2 using anti-GRHL2 antibody (A04120-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human T-47D whole cell lysates, Lane 2: human RT4 whole cell lysates, Lane 3: human Hacat whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRHL2 antigen affinity purified polyclonal antibody (Catalog # A04120-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRHL2 at approximately 71 kDa. The expected band size for GRHL2 is at 71 kDa.
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