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ProductsBack/GRIN2B / NMDAR2B / NR2B Antibody

GRIN2B / NMDAR2B / NR2B Antibody

LS-C16903
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
Product group Antibodies
TargetGRIN2B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GRIN2B / NMDAR2B / NR2B Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Suitable for use in Immunohistochemistry (4% paraformaldehyde & 0.125% glutaraldehyde fixed tissue), Immunocytochemistry, and Western Blot. Immunohistochemistry: 2.5-5 ug/ml. Immunocytochemistry: 2.5-5.0 ug/ml. Western blot: 1-5 ug/ml.. ICC (2.5 - 5 µg/ml), IHC (2.5 - 5 µg/ml), WB (1 - 5 µg/ml) Suitable for use in Immunohistochemistry (4% paraformaldehyde & 0.125% glutaraldehyde fixed tissue), Immunocytochemistry, and Western Blot. Immunohistochemistry: 2.5-5 ug/ml. Immunocytochemistry: 2.5-5.0 ug/ml. Western blot: 1-5 ug/ml.
  • Applications
    Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2904
  • Target name
    GRIN2B
  • Target description
    glutamate ionotropic receptor NMDA type subunit 2B
  • Target synonyms
    DEE27; EIEE27; GluN2B; GluN2B(alt_5'UTR); glutamate [NMDA] receptor subunit epsilon-2; glutamate receptor ionotropic, NMDA 2B; glutamate receptor subunit epsilon-2; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; hNR3; MRD6; NMDAR2B; N-methyl D-aspartate receptor subtype 2B; N-methyl-D-aspartate receptor subunit 3; NR2B; NR3
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NMDAR2B using anti-NMDAR2B antibody (PB9373). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMDAR2B antigen affinity purified polyclonal antibody (Catalog # PB9373) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMDAR2B at approximately 180-190 kDa. The expected band size for NMDAR2B is at 166 kDa.
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