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ProductsBack/Goat anti-NMDAR2B / GRIN2B

Goat anti-NMDAR2B / GRIN2B

EB07294
Everest Biotech
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
TargetGRIN2B
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-NMDAR2B / GRIN2B Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Immunofluorescence: Strong expression of the protein seen in the cytoskeleton/ cytoplasm of HeLa cells. Recommended concentration: 10microg/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID2904
  • Target name
    GRIN2B
  • Target description
    glutamate ionotropic receptor NMDA type subunit 2B
  • Target synonyms
    DEE27, EIEE27, GluN2B, MRD6, NMDAR2B, NR2B, NR3, hNR3, glutamate receptor ionotropic, NMDA 2B, GluN2B(alt_5'UTR), N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000825.1. GeneIDs all Nonhuman: 14812 (mouse); 24410 (rat);. Purification: Antigen affinity purified. Names and symbols: GRIN2B; NMDAR2B; glutamate receptor, ionotropic, N-methyl D-aspartate 2B; HGNC:4586; NR2B; hNR3 ; N-methyl-D-aspartate receptor subunit 2B
  • Reactivity Supplier
    Human, Mouse, Rat, Dog
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of NMDAR2B using anti-NMDAR2B antibody (PB9373). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: rat C6 whole cell lysates, Lane 3: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NMDAR2B antigen affinity purified polyclonal antibody (Catalog # PB9373) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for NMDAR2B at approximately 180-190 kDa. The expected band size for NMDAR2B is at 166 kDa.
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