ICC/IF analysis of human cells using GTX22776 GRK1 antibody [D11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC
![ICC/IF analysis of murine cells using GTX22776 GRK1 antibody [D11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX22776/GTX22776_2020_ICC-IF_w_23060620_906.webp "ICC/IF analysis of murine cells using GTX22776 GRK1 antibody [D11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber")
![ICC/IF analysis of HeLa cells using GTX22776 GRK1 antibody [D11]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX22776/GTX22776_431_ICC-IF_w_23060620_354.webp "ICC/IF analysis of HeLa cells using GTX22776 GRK1 antibody [D11]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber")
![IHC-Fr analysis of bovine retina tissue using GTX22776 GRK1 antibody [D11].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22776/GTX22776_957_IHC-Fr_w_23060620_950.webp "IHC-Fr analysis of bovine retina tissue using GTX22776 GRK1 antibody [D11].")
ICC/IF analysis of human cells using GTX22776 GRK1 antibody [D11]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:20 overnight at 4oC
GRK1 antibody [D11]
GTX22776
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityBovine, Human, Mouse
TargetGRK1
Overview
- SupplierGeneTex
- Product NameGRK1 antibody [D11]
- Delivery Days Customer9
- Antibody SpecificityThis antibody does not cross-react with other G protein-coupled kinases. It recognizes an N-terminal epitope of human GRK1 which is conserved in both GRK1a and GRK1b.
- Application Supplier NoteWB: 1:1,000. ICC/IF: 1:20-1:200. IHC-Fr: 1:1,000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDD11
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6011
- Target nameGRK1
- Target descriptionG protein-coupled receptor kinase 1
- Target synonymsGPRK1; rhodopsin kinase GRK1; RHOK; RK
- HostMouse
- IsotypeIgG1
- Protein IDQ15835
- Protein NameRhodopsin kinase
- Scientific DescriptionThis gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]
- ReactivityBovine, Human, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203