ICC/IF analysis of human cells using GTX22775 GRK1 antibody [G8]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber
![ICC/IF analysis of murine cells using GTX22775 GRK1 antibody [G8]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX22775/GTX22775_2018_ICC-IF_w_23060620_744.webp "ICC/IF analysis of murine cells using GTX22775 GRK1 antibody [G8]. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber")
![ICC/IF analysis of MCF-7 cells using GTX22775 GRK1 antibody [G8]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature](https://www.genetex.com/upload/website/prouct_img/normal/GTX22775/GTX22775_429_ICC-IF_w_23060620_991.webp "ICC/IF analysis of MCF-7 cells using GTX22775 GRK1 antibody [G8]. Panel e is a no primary antibody control. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : 4% paraformaldehyde Permeabilization : 0.1% Triton X-100 for 10 minute Dilution : 2 microg/ml in 0.1% BSA and incubated for 3 hours at room temperature")
![ICC/IF analysis of HeLa cells using GTX22775 GRK1 antibody [G8]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber](https://www.genetex.com/upload/website/prouct_img/normal/GTX22775/GTX22775_430_ICC-IF_w_23060620_315.webp "ICC/IF analysis of HeLa cells using GTX22775 GRK1 antibody [G8]. Cells were probed without (left) or with(right) an antibody. Green : Primary antibody Blue : Nuclei Red : Actin Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:200 and incubated overnight in a humidified chamber")
![IHC-Fr analysis of bovine retina tissue using GTX22775 GRK1 antibody [G8].](https://www.genetex.com/upload/website/prouct_img/normal/GTX22775/GTX22775_956_IHC-Fr_w_23060620_615.webp "IHC-Fr analysis of bovine retina tissue using GTX22775 GRK1 antibody [G8].")
ICC/IF analysis of human cells using GTX22775 GRK1 antibody [G8]. Fixation : Formalin Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minute Dilution : 1:100 incubated overnight in a humidified chamber
GRK1 antibody [G8]
GTX22775
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
ReactivityBovine, Canine, Chicken, Human, Mouse, Rat
TargetGRK1
Overview
- SupplierGeneTex
- Product NameGRK1 antibody [G8]
- Delivery Days Customer9
- Antibody SpecificityThis antibody recognizes an epitope in the C-terminal region of GRK1a.
- Application Supplier NoteWB: 1 microg/ml. ICC/IF: 2-3 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone IDG8
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID6011
- Target nameGRK1
- Target descriptionG protein-coupled receptor kinase 1
- Target synonymsGPRK1; rhodopsin kinase GRK1; RHOK; RK
- HostMouse
- IsotypeIgG1
- Protein IDQ15835
- Protein NameRhodopsin kinase
- Scientific DescriptionThis gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kinase subfamily of the Ser/Thr protein kinase family. The protein phosphorylates rhodopsin and initiates its deactivation. Defects in GRK1 are known to cause Oguchi disease 2 (also known as stationary night blindness Oguchi type-2). [provided by RefSeq, Jul 2008]
- ReactivityBovine, Canine, Chicken, Human, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203