Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101682/GTX101682_43691_20190906_WB_w_23060100_658.webp "Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101682/GTX101682_43691_20211203_WB_KO_watermark_w_23060100_949.webp "Wild-type (WT) and GRK2 knockout (KO) 293T cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![GRK2 antibody [C2C3], C-term detects GRK2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GRK2 stained by GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX101682/GTX101682_43530_20211126_ICC_IF_w_23060100_446.webp "GRK2 antibody [C2C3], C-term detects GRK2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: GRK2 stained by GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000. Blue: Fluoroshield with DAPI (GTX30920).")
![Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 μg of GRK2 antibody [C2C3], C-term (GTX101682). Western blot analysis was performed using GRK2 antibody [C2C3], C-term (GTX101682). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101682/GTX101682_39995_20150522_IP_w_23060100_312.webp "Immunoprecipitation of GRK2 protein from Jurkat whole cell extracts using 5 μg of GRK2 antibody [C2C3], C-term (GTX101682). Western blot analysis was performed using GRK2 antibody [C2C3], C-term (GTX101682). EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX101682/GTX101682_39995_20180831_WB_B_w_23060100_715.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with GRK2 antibody [C2C3], C-term (GTX101682) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GRK2 antibody [C2C3], C-term
GTX101682
ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetGRK2
Overview
- SupplierGeneTex
- Product NameGRK2 antibody [C2C3], C-term
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration0.82 mg/ml
- ConjugateUnconjugated
- Gene ID156
- Target nameGRK2
- Target descriptionG protein-coupled receptor kinase 2
- Target synonymsADRBK1, BARK1, BETA-ARK1, beta-adrenergic receptor kinase 1, adrenergic beta receptor kinase 1, beta-ARK-1
- HostRabbit
- IsotypeIgG
- Protein IDP25098
- Protein NameBeta-adrenergic receptor kinase 1
- Scientific DescriptionThe product of this gene phosphorylates the beta-2-adrenergic receptor and appears to mediate agonist-specific desensitization observed at high agonist concentrations. This protein is an ubiquitous cytosolic enzyme that specifically phosphorylates the activated form of the beta-adrenergic and related G-protein-coupled receptors. Abnormal coupling of beta-adrenergic receptor to G protein is involved in the pathogenesis of the failing heart. [provided by RefSeq]
- ReactivityHuman, Mouse
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Su VY, Chen WC, Yu WK, et al. Nintedanib Regulates GRK2 and CXCR2 to Reduce Neutrophil Recruitment in Endotoxin-Induced Lung Injury. Int J Mol Sci. 2021,22(18). doi: 10.3390/ijms22189898Read this paper
- Barkaway A, Rolas L, Joulia R, et al. Age-related changes in the local milieu of inflamed tissues cause aberrant neutrophil trafficking and subsequent remote organ damage. Immunity. 2021,54(7):1494-1510.e7. doi: 10.1016/j.immuni.2021.04.025Read this paper
- Huang P, Lu X, Yuan B, et al. Astragaloside IV alleviates E. coli-caused peritonitis via upregulation of neutrophil influx to the site of infection. Int Immunopharmacol. 2016,39:377-382. doi: 10.1016/j.intimp.2016.08.011Read this paper
- Dasu MR, Ramirez SR, La TD, et al. Crosstalk between adrenergic and toll-like receptors in human mesenchymal stem cells and keratinocytes: a recipe for impaired wound healing. Stem Cells Transl Med. 2014,3(6):745-59. doi: 10.5966/sctm.2013-0200Read this paper
Datasheet
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