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Western blot All lanes: ADRBK1 antibody at 3ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa
Western blot All lanes: ADRBK1 antibody at 3ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa
Western blot All lanes: ADRBK1 antibody at 3ug/ml + Mouse brain tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 80 kDa Observed band size: 80 kDa

GRK2 Antibody

CSB-PA001394DSR1HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman, Mouse
TargetGRK2
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Overview

  • Supplier
    Cusabio
  • Product Name
    GRK2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID156
  • Target name
    GRK2
  • Target description
    G protein-coupled receptor kinase 2
  • Target synonyms
    ADRBK1, BARK1, BETA-ARK1, beta-adrenergic receptor kinase 1, adrenergic beta receptor kinase 1, beta-ARK-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP25098
  • Protein Name
    Beta-adrenergic receptor kinase 1
  • Scientific Description
    Specifically phosphorylates the agonist-occupied form of the beta-adrenergic and closely related receptors, probably inducing a desensitization of them. Key regulator of LPAR1 signaling. Competes with RALA for binding to LPAR1 thus affecting the signaling properties of the receptor. Desensitizes LPAR1 and LPAR2 in a phosphorylation-independent manner.
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GRK2 using anti-GRK2 antibody (A01473-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat spleen tissue lysates, Lane 2: rat stomach tissue lysates, Lane 3: mouse lung tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: mouse pancreas tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRK2 antigen affinity purified polyclonal antibody (Catalog # A01473-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRK2 at approximately 79KD. The expected band size for GRK2 is at 79KD.
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