
ICC/IF analysis of HeLa cells using GTX48633 Grp78 antibody. Green : primary antibody Red : Tubulin Blue : DAPI Dilution : 5 μg/ml
Grp78 antibody
GTX48633
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityBovine, C. Elegans, Chicken, Drosophila, Human, Mouse, Primate, Rat, Sheep, Zebra Fish
TargetHSPA5
Overview
- SupplierGeneTex
- Product NameGrp78 antibody
- Delivery Days Customer9
- Application Supplier NoteWB: 0.5 microg/ml. ICC/IF: 1:50. FCM: 1:150. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID3309
- Target nameHSPA5
- Target descriptionheat shock protein family A (Hsp70) member 5
- Target synonymsBIP, GRP78, HEL-S-89n, endoplasmic reticulum chaperone BiP, 78 kDa glucose-regulated protein, HSP70 family protein 5, binding-immunoglobulin protein, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, epididymis secretory sperm binding protein Li 89n, glucose-regulated protein, 78kDa, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), heat shock protein 70 family protein 5, heat shock protein family A member 5, immunoglobulin heavy chain-binding protein
- HostRabbit
- IsotypeIgG
- Protein IDP11021
- Protein NameEndoplasmic reticulum chaperone BiP
- Scientific DescriptionWhen Chinese hamster K12 cells are starved of glucose, the synthesis of several proteins, called glucose-regulated proteins (GRPs), is markedly increased. Hendershot et al. (1994) [PubMed 8020977] pointed out that one of these, GRP78 (HSPA5), also referred to as immunoglobulin heavy chain-binding protein (BiP), is a member of the heat-shock protein-70 (HSP70) family and is involved in the folding and assembly of proteins in the endoplasmic reticulum (ER). Because so many ER proteins interact transiently with GRP78, it may play a key role in monitoring protein transport through the cell.[supplied by OMIM]
- ReactivityBovine, C. Elegans, Chicken, Drosophila, Human, Mouse, Primate, Rat, Sheep, Zebra Fish
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
References
- Inhibition of endoplasmic reticulum stress-related autophagy attenuates MCLR-induced apoptosis in zebrafish testis and mouse TM4 cells. Wu Z et al., 2021 Sep 15, Ecotoxicol Environ SafRead this paper
- Thrombospondin expression in myofibers stabilizes muscle membranes. Vanhoutte D et al., 2016 Sep 26, ElifeRead this paper













![Grp78 antibody [HL1815] detects Grp78 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. Grp78 stained by Grp78 antibody [HL1815] (GTX637540) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX637540/GTX637540_T-44816_20221118_IHC-P_R_22112219_613.webp)