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GRP78 BiP polyclonal antibody

ORB1725583
Biorbyt
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHSPA5
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Overview

  • Supplier
    Biorbyt
  • Product Name
    GRP78 BiP polyclonal antibody
  • Delivery Days Customer
    10
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB: 1/500-1/1000 IHC: 1/50-1/100 IF: 1/50-1/200 ELISA: 1/10000 ELISA, ICC, IHC-Fr, IHC-P, WB
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3309
  • Target name
    HSPA5
  • Target description
    heat shock protein family A (Hsp70) member 5
  • Target synonyms
    BIP, GRP78, HEL-S-89n, endoplasmic reticulum chaperone BiP, 78 kDa glucose-regulated protein, HSP70 family protein 5, binding-immunoglobulin protein, endoplasmic reticulum lumenal Ca(2+)-binding protein grp78, epididymis secretory sperm binding protein Li 89n, glucose-regulated protein, 78kDa, heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa), heat shock protein 70 family protein 5, heat shock protein family A member 5, immunoglobulin heavy chain-binding protein
  • Host
    Rabbit
  • Protein IDP11021
  • Protein Name
    Endoplasmic reticulum chaperone BiP
  • Scientific Description
    GRP78 BiP polyclonal antibody
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Image provided by One World Lab validation program. SMMC7721 cells probed with Rabbit Anti-GRP78 Polyclonal Antibody (bs-1219R) at 1:100 for 60 minutes at room temperature followed by Goat Anti-Rabbit IgG (H+L) Alexa Fluor 488 Conjugated secondary antibody.
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Figure 1. Western blot analysis of GRP78/BIP/HSPA5 using anti-GRP78/BIP/HSPA5 antibody (A00955). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human T-47D whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat brain tissue lysates, Lane 7: mouse liver tissue lysates, Lane 8: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GRP78/BIP/HSPA5 antigen affinity purified polyclonal antibody (Catalog # A00955) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GRP78/BIP/HSPA5 at approximately 78 kDa. The expected band size for GRP78/BIP/HSPA5 is at 78 kDa.
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