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GSK3A antibody

70R-17614

Product group Antibodies

Overview

Supplier
Biosynth
Product Name
GSK3A antibody
Delivery Days Customer
11
Certification
Research Use Only
UNSPSC
12352203

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Figure 1. Western blot analysis of GSK3 alpha using anti-GSK3 alpha antibody (A03152-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: mouse testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 alpha antigen affinity purified polyclonal antibody (Catalog # A03152-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 alpha at approximately 51KD. The expected band size for GSK3 alpha is at 51KD.

Product group Antibodies

Anti-GSK3 alpha/GSK3A Antibody Picoband(r)A03152-2-CARRIER-FREE

ApplicationsWestern Blot

ReactivityHuman, Mouse, Rat

TargetGSK3A

SizePrice

Product group Antibodies

GSK3 alpha AntibodyABX012858

ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry

SizePrice

Product group Antibodies

Anti-GSK3A Antibody144-00645

ApplicationsWestern Blot

ReactivityHuman, Mouse, Rat

TargetGSK3A

SizePrice

Product group Antibodies

GSK3A Polyclonal AntibodyCAC14772

ApplicationsImmunoFluorescence, Western Blot, ELISA

TargetGSK3A

SizePrice

Product group Antibodies

GSK3alpha AntibodyCAB0645

ReactivityHuman

TargetGSK3A

SizePrice

$RSC96 cells probed with Rabbit Anti-GSK3 Alpha/Beta(Tyr279+Tyr216) Polyclonal Antibody (bs-2073R), 3ug,for 20 minutes followed by incubation with Goat Anti-Rabbit IgG FITC conjugated secondary (bs-0295G-FITC) at 1:200 (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).\n$

Product group Antibodies

phospho-GSK3 Alpha + Beta (Tyr279+Tyr216) Polyclonal AntibodyBS-2073R

ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep

TargetGSK3A