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Western Blot analysis of Jurkat cells using GSK3alpha Polyclonal Antibody
Western Blot analysis of Jurkat cells using GSK3alpha Polyclonal Antibody
Western Blot analysis of Jurkat cells using GSK3alpha Polyclonal Antibody

GSK3A Antibody

CSB-PA002836
Cusabio
ApplicationsImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGSK3A
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Overview

  • Supplier
    Cusabio
  • Product Name
    GSK3A Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2931
  • Target name
    GSK3A
  • Target description
    glycogen synthase kinase 3 alpha
  • Target synonyms
    glycogen synthase kinase-3 alpha; GSK-3 alpha; serine/threonine-protein kinase GSK3A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP49840
  • Protein Name
    Glycogen synthase kinase-3 alpha
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GSK3 alpha using anti-GSK3 alpha antibody (A03152-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysate, Lane 2: rat testis tissue lysate, Lane 3: mouse testis tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 alpha antigen affinity purified polyclonal antibody (Catalog # A03152-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 alpha at approximately 51KD. The expected band size for GSK3 alpha is at 51KD.
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