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ProductsBack/GSK3B / GSK3 Beta Antibody

GSK3B / GSK3 Beta Antibody

LS-C814213
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetGSK3B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    GSK3B / GSK3 Beta Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Applications should be user optimized.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:100 - 1:200), WB (1:1000 - 1:2000) Applications should be user optimized.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID2932
  • Target name
    GSK3B
  • Target description
    glycogen synthase kinase 3 beta
  • Target synonyms
    glycogen synthase kinase-3 beta, GSK-3 beta, serine/threonine-protein kinase GSK3B
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of GSK3 beta/GSK3B using anti-GSK3 beta/GSK3B antibody (A00791-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human U-87MG whole cell lysates, Lane 3: rat PC-12 whole cell lysates, Lane 4: rat C6 whole cell lysates, Lane 5: mouse RAW264.7 whole cell lysates, Lane 6: mouse Neuro-2a whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSK3 beta/GSK3B antigen affinity purified polyclonal antibody (Catalog # A00791-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSK3 beta/GSK3B at approximately 46 kDa. The expected band size for GSK3 beta/GSK3B is at 46 kDa.
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