Immunohistochemical analysis of paraffin-embedded H661 xenograft, using GSK3 beta(GTX111192) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
![GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm and cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta stained by GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111192/GTX111192_44384_20220408_ICC_IF_w_23060500_975.webp "GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm and cell membrane by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta stained by GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920). Scale bar= 10μm.")
![Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111192/GTX111192_40051_20160526_WB_shRNA_watermark_w_23060500_458.webp "Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111192/GTX111192_40051_20170811_WB_w_23060500_559.webp "Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.")
![GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.](https://www.genetex.com/upload/website/prouct_img/normal/GTX111192/GTX111192_40051_20160325_IFA_w_23060500_994.webp "GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm by immunofluorescent analysis. Sample: SK-N-SH cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: GSK3 beta protein stained by GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:400. Blue: Hoechst 33342 staining. Scale bar = 10 μm.")
![GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min](https://www.genetex.com/upload/website/prouct_img/normal/GTX111192/GTX111192_40051_20160127_IHC-P_R_w_23060500_291.webp "GSK3 beta antibody [C1C3] detects GSK3 beta protein at cytoplasm in rat brain by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. GSK3 beta antibody [C1C3] (GTX111192) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min")
Immunohistochemical analysis of paraffin-embedded H661 xenograft, using GSK3 beta(GTX111192) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy? (EDTA based, pH 8.0) buffer, 15min
GSK3 beta antibody [C1C3]
GTX111192
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetGSK3B
Overview
- SupplierGeneTex
- Product NameGSK3 beta antibody [C1C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.57 mg/ml
- ConjugateUnconjugated
- Gene ID2932
- Target nameGSK3B
- Target descriptionglycogen synthase kinase 3 beta
- Target synonymsglycogen synthase kinase-3 beta, GSK-3 beta, serine/threonine-protein kinase GSK3B
- HostRabbit
- IsotypeIgG
- Protein IDP49841
- Protein NameGlycogen synthase kinase-3 beta
- Scientific DescriptionGlycogen synthase kinase-3 (GSK3) is a proline-directed serine-threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase (see GYS1, MIM 138570). Two isoforms, alpha (GSK3A; MIM 606784) and beta, show a high degree of amino acid homology (Stambolic and Woodgett, 1994 [PubMed 7980435]). GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation (Plyte et al., 1992 [PubMed 1333807]).[supplied by OMIM]
- ReactivityHuman, Mouse, Rat
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Yang L, Lei JF, Ouyang JY, et al. Effect of Neurorepair for Motor Functional Recovery Enhanced by Total Saponins From Trillium tschonoskii Maxim. Treatment in a Rat Model of Focal Ischemia. Front Pharmacol. 2021,12:763181. doi: 10.3389/fphar.2021.763181Read this paper
- Jadhao M, Tsai EM, Yang HC, et al. The Long-Term DEHP Exposure Confers Multidrug Resistance of Triple-Negative Breast Cancer Cells through ABC Transporters and Intracellular ROS. Antioxidants (Basel). 2021,10(6). doi: 10.3390/antiox10060949Read this paper
- Samuel M, Fonseka P, Sanwlani R, et al. Oral administration of bovine milk-derived extracellular vesicles induces senescence in the primary tumor but accelerates cancer metastasis. Nat Commun. 2021,12(1):3950. doi: 10.1038/s41467-021-24273-8Read this paper
- Chang YX, Lin YF, Chen CL, et al. Chaperonin-Containing TCP-1 Promotes Cancer Chemoresistance and Metastasis through the AKT-GSK3β-β-Catenin and XIAP-Survivin Pathways. Cancers (Basel). 2020,12(12). doi: 10.3390/cancers12123865Read this paper
- Pan CH, Chen SY, Wang JY, et al. Sclareol ameliorated ERCC1-mediated cisplatin resistance in A549 human lung adenocarcinoma cells and a murine xenograft tumor model by suppressing AKT-GSK3β-AP1/Snail and JNK-AP1 pathways. Chem Biol Interact. 2020,332:109304. doi: 10.1016/j.cbi.2020.109304Read this paper
- Khan K, Yu B, Kiwan C, et al. The Role of Wnt/β-Catenin Pathway Mediators in Aortic Valve Stenosis. Front Cell Dev Biol. 2020,8:862. doi: 10.3389/fcell.2020.00862Read this paper
- Zhan Y, Li MZ, Yang L, et al. The three-phase enriched environment paradigm promotes neurovascular restorative and prevents learning impairment after ischemic stroke in rats. Neurobiol Dis. 2020,146:105091. doi: 10.1016/j.nbd.2020.105091Read this paper
- Zuo Z, Ji S, He L, et al. LncRNA TTN-AS1/miR-134-5p/PAK3 axis regulates the radiosensitivity of human large intestine cancer cells through the P21 pathway and AKT/GSK-3β/β-catenin pathway. Cell Biol Int. 2020,44(11):2284-2292. doi: 10.1002/cbin.11436Read this paper
- Huang JM, Huang FI, Yang CR. Moscatilin Ameliorates Tau Phosphorylation and Cognitive Deficits in Alzheimer's Disease Models. J Nat Prod. 2019,82(7):1979-1988. doi: 10.1021/acs.jnatprod.9b00375Read this paper
- Liu J, Chen W, Zhang H, et al. miR-214 targets the PTEN-mediated PI3K/Akt signaling pathway and regulates cell proliferation and apoptosis in ovarian cancer. Oncol Lett. 2017,14(5):5711-5718. doi: 10.3892/ol.2017.6953Read this paper
Datasheet
Related products
Product group Antibodies
ApplicationsELISA
ReactivityHuman
TargetGSK3B
- SizePrice
Product group Antibodies
GSK3B Polyclonal AntibodyCAC13750
ApplicationsImmunoFluorescence, Western Blot, ELISA
ReactivityMouse
TargetGSK3B
- SizePrice
Product group Antibodies
References
GSK-3 Beta Polyclonal AntibodyBS-0028R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGSK3B
- SizePrice
Product group Antibodies
GSK3B / GSK3 Beta Antibody (clone 4C3)LS-C767028
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGSK3B
- SizePrice
Product group Antibodies
anti-Phospho-GSK-3beta (Ser9), Rabbit Monoclonal (RM453)REV-31-1345-00
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetGSK3B
- SizePrice
Product group Antibodies
References
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityDrosophila, Human
TargetGSK3B
- SizePrice
![WB analysis of (1) HeLa cell, (2) 3T3 cell, (3) rat brain tissue lysates using GTX33980 GSK3 beta antibody [1A6]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX33980/GTX33980_20200622_WB_447_w_23060801_590.webp)
Product group Antibodies
GSK3 beta antibody [1A6]GTX33980
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGSK3B
- SizePrice
![WB analysis of (1) HeLa cell, (2) 3T3 cell, (3) rat brain tissue lysates using GTX33981 GSK3 beta antibody [2C6]. Dilution : 1:1000](https://www.genetex.com/upload/website/prouct_img/normal/GTX33981/GTX33981_20200622_WB_448_w_23060801_439.webp)
Product group Antibodies
GSK3 beta antibody [2C6]GTX33981
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse, Rat
TargetGSK3B
- SizePrice