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Western blot All lanes: GSTA4 antibody at 8ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 26, 15 kDa Observed band size: 26 kDa
Western blot All lanes: GSTA4 antibody at 8ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 26, 15 kDa Observed band size: 26 kDa
Western blot All lanes: GSTA4 antibody at 8ug/ml + 293T whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 26, 15 kDa Observed band size: 26 kDa

GSTA4 Antibody

CSB-PA009973ESR1HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetGSTA4
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Overview

  • Supplier
    Cusabio
  • Product Name
    GSTA4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID2941
  • Target name
    GSTA4
  • Target description
    glutathione S-transferase alpha 4
  • Target synonyms
    glutathione S-alkyltransferase A4; glutathione S-aralkyltransferase A4; glutathione S-aryltransferase A4; glutathione S-transferase A4; glutathione S-transferase A4-4; glutathione transferase A4-4; GST class-alpha member 4; GSTA4-4; GTA4; S-(hydroxyalkyl)glutathione lyase A4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15217
  • Protein Name
    Glutathione S-transferase A4
  • Scientific Description
    Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles. This isozyme has a high catalytic efficiency with 4-hydroxyalkenals such as 4-hydroxynonenal (4-HNE).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of GSTA4 using anti-GSTA4 antibody (A03699-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates, Lane 2: human SH-SY5Y lysates, Lane 3: human HEPG2 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: monkey skeletal muscle tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GSTA4 antigen affinity purified polyclonal antibody (Catalog # A03699-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GSTA4 at approximately 26KD. The expected band size for GSTA4 is at 26KD.
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