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ProductsBack/GZMH Antibody - N-terminal region (OASG03200)

GZMH Antibody - N-terminal region (OASG03200)

OASG03200
Aviva Systems Biology
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetGzmb
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Overview

  • Supplier
    Aviva Systems Biology
  • Product Name
    GZMH Antibody - N-terminal region (OASG03200)
  • Delivery Days Customer
    23
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Format
    Liquid. 1 mg/mL in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
  • Gene ID14939
  • Target name
    Gzmb
  • Target description
    granzyme B
  • Target synonyms
    CCP-1/C11, CCP1, Ctla-1, Ctla1, GZB, granzyme B(G,H), cytotoxic cell protease 1, fragmentin-2
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Granzyme B using anti-Granzyme B antibody (A00353-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse thymus tissue lysates, Lane 2: mouse liver tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Granzyme B antigen affinity purified polyclonal antibody (Catalog # A00353-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Granzyme B at approximately 28KD. The expected band size for Granzyme B is at 28KD.
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