Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Bioss antibody against H3K27me3 (Cat. No. bs-53122R) diluted 1:500 in TBS-Tween containing 5% skimmed milk.
Western blot was performed on whole cell (25 μg, lane 1) and histone extracts (15 μg, lane 2) from HeLa cells, and on 1 μg of recombinant histone H2A, H2B, H3 and H4 (lane 3, 4, 5 and 6, respectively) using the Bioss antibody against H3K27me3 (Cat. No. bs-53122R) diluted 1:500 in TBS-Tween containing 5% skimmed milk.
H3K27me3 Polyclonal Antibody
BS-53122R
ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
Product group Antibodies
ReactivityDrosophila, Human, Mouse, Plant, Rat, Schistosoma japonicum, Zebra Fish, Other Species
TargetH3C1
Overview
- SupplierBioss
- Product NameH3K27me3 Polyclonal Antibody
- Delivery Days Customer16
- ApplicationsDot Blot, ImmunoFluorescence, Western Blot, ChIP Chromatin ImmunoPrecipitation, ELISA
- Applications SupplierWB(1:300-5000), ELISA(1:500-1000), IF(), dot-blot(), ChIP-seq()
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.45 ug/ul
- ConjugateUnconjugated
- Gene ID8350
- Target nameH3C1
- Target descriptionH3 clustered histone 1
- Target synonymsH3/A, H3C10, H3C11, H3C12, H3C2, H3C3, H3C4, H3C6, H3C7, H3C8, H3FA, HIST1H3A, histone H3.1, H3 histone family, member A, histone 1, H3a, histone H3/a, histone cluster 1 H3 family member a, histone cluster 1, H3a
- HostRabbit
- Protein IDP68431
- Protein NameHistone H3.1
- ReactivityDrosophila, Human, Mouse, Plant, Rat, Schistosoma japonicum, Zebra Fish, Other Species
- Storage Instruction-20°C
- UNSPSC12352203
Datasheet
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References
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References
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