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HADHB Rabbit pAb

A5716
ABclonal
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHADHB
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Overview

  • Supplier
    ABclonal
  • Product Name
    HADHB Rabbit pAb
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3032
  • Target name
    HADHB
  • Target description
    hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta
  • Target synonyms
    ECHB, MSTP029, MTPB, MTPD, MTPD2, TP-BETA, trifunctional enzyme subunit beta, mitochondrial, 2-enoyl-Coenzyme A (CoA) hydratase, beta subunit, 3-ketoacyl-Coenzyme A (CoA) thiolase of mitochondrial trifunctional protein, beta subunit, acetyl-CoA acyltransferase, beta-ketothiolase, hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit, hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP55084
  • Protein Name
    Trifunctional enzyme subunit beta, mitochondrial
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HADHB using anti-HADHB antibody (A04776-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: rat heart tissue lysates, Lane 5: rat skeletal muscle tissue lysates, Lane 6: mouse heart tissue lysates, Lane 7: mouse skeletal muscle tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HADHB antigen affinity purified polyclonal antibody (Catalog # A04776-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HADHB at approximately 45 kDa. The expected band size for HADHB is at 51 kDa.
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TargetHADHB
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Lane 1: Siha cell lysates probed with HADHB Polyclonal Antibody, Unconjugated (bs-5065R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with HADHB antibody [N1], N-term (GTX100302) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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Immunohistochemical staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
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