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ProductsBack/HAO1 Antibody (clone OTI5F4)

HAO1 Antibody (clone OTI5F4)

LS-C172430
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot
Product group Antibodies
TargetHAO1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HAO1 Antibody (clone OTI5F4)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot
  • Applications Supplier
    Flo (1:100), WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI5F4
  • Concentration
    0.49 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID54363
  • Target name
    HAO1
  • Target description
    hydroxyacid oxidase 1
  • Target synonyms
    (S)-2-hydroxy-acid oxidase; glycolate oxidase 1; GOX; GOX1; HAOX1; hydroxyacid oxidase (glycolate oxidase) 1; hydroxyacid oxidase 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HAO1 using anti-HAO1 antibody (A09159-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAO1 antigen affinity purified polyclonal antibody (Catalog # A09159-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAO1 at approximately 41 kDa. The expected band size for HAO1 is at 41 kDa.
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