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Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.
Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.
Western blot analysis of 1) Mouse Liver Tissue, 2) Rat Liver Tissue using HAO1 Monoclonal Antibody.

HAO1 Monoclonal Antibody

CSB-MA080172
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityMouse, Rat
TargetHAO1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HAO1 Monoclonal Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID54363
  • Target name
    HAO1
  • Target description
    hydroxyacid oxidase 1
  • Target synonyms
    GO, GOX, GOX1, HAOX1, 2-Hydroxyacid oxidase 1, (S)-2-hydroxy-acid oxidase, glycolate oxidase 1, glyoxylate oxidase, hydroxyacid oxidase (glycolate oxidase) 1
  • Host
    Mouse
  • Isotype
    IgG
  • Protein IDQ9UJM8
  • Protein Name
    Hydroxyacid oxidase 1
  • Reactivity
    Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HAO1 using anti-HAO1 antibody (A09159-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAO1 antigen affinity purified polyclonal antibody (Catalog # A09159-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAO1 at approximately 41 kDa. The expected band size for HAO1 is at 41 kDa.
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