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IHC-P analysis of human liver tissue using GTX33987 HAO1 antibody [Mix]. Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98oC, 20min) Dilution : 1:200
IHC-P analysis of human liver tissue using GTX33987 HAO1 antibody [Mix]. Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98oC, 20min) Dilution : 1:200
IHC-P analysis of human liver tissue using GTX33987 HAO1 antibody [Mix]. Negative control (the lower left coner) was secondary antibody only. Antigen retrieval : Sodium citrate pH6.0 was used for antibody retrieval (>98oC, 20min) Dilution : 1:200

HAO1 antibody [Mix]

GTX33987
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHAO1
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Overview

  • Supplier
    GeneTex
  • Product Name
    HAO1 antibody [Mix]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000-1:2000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Clone ID
    Mix
  • Conjugate
    Unconjugated
  • Gene ID54363
  • Target name
    HAO1
  • Target description
    hydroxyacid oxidase 1
  • Target synonyms
    GO, GOX, GOX1, HAOX1, 2-Hydroxyacid oxidase 1, (S)-2-hydroxy-acid oxidase, glycolate oxidase 1, glyoxylate oxidase, hydroxyacid oxidase (glycolate oxidase) 1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9UJM8
  • Protein Name
    Hydroxyacid oxidase 1
  • Scientific Description
    This gene is one of three related genes that have 2-hydroxyacid oxidase activity yet differ in encoded protein amino acid sequence, tissue expression and substrate preference. Subcellular location of the encoded protein is the peroxisome. Specifically, this gene is expressed primarily in liver and pancreas and the encoded protein is most active on glycolate, a two-carbon substrate. The protein is also active on 2-hydroxy fatty acids. The transcript detected at high levels in pancreas may represent an alternatively spliced form or the use of a multiple near-consensus upstream polyadenylation site. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HAO1 using anti-HAO1 antibody (A09159-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAO1 antigen affinity purified polyclonal antibody (Catalog # A09159-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAO1 at approximately 41 kDa. The expected band size for HAO1 is at 41 kDa.
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