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Western Blot Positive WB detected in: MCF-7 whole cell lysate, Rat spleen tissue All lanes: HAT1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 40 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Rat spleen tissue All lanes: HAT1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 40 kDa Observed band size: 50 kDa
Western Blot Positive WB detected in: MCF-7 whole cell lysate, Rat spleen tissue All lanes: HAT1 antibody at 3microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 50, 40 kDa Observed band size: 50 kDa

HAT1 Antibody

CSB-PA010143EA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA
Product group Antibodies
ReactivityHuman, Rat
TargetHAT1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HAT1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8520
  • Target name
    HAT1
  • Target description
    histone acetyltransferase 1
  • Target synonyms
    KAT1, histone acetyltransferase type B catalytic subunit
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO14929
  • Protein Name
    Histone acetyltransferase type B catalytic subunit
  • Scientific Description
    Acetylates soluble but not nucleosomal histone H4 at Lys-5 (H4K5ac) and Lys-12 (H4K12ac) and, to a lesser extent, acetylates histone H2A at Lys-5 (H2AK5ac). Has intrinsic substrate specificity that modifies lysine in recognition sequence GXGKXG. May be involved in nucleosome assembly during DNA replication and repair as part of the histone H3.1 and H3.3 complexes. May play a role in DNA repair in response to free radical damage.
  • Reactivity
    Human, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of KAT1/HAT1 using anti-KAT1/HAT1 antibody (A03596-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat stomach tissue lysates, Lane 2: rat testis tissue lysates, Lane 3: mouse testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KAT1/HAT1 antigen affinity purified polyclonal antibody (Catalog # A03596-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for KAT1/HAT1 at approximately 50 kDa. The expected band size for KAT1/HAT1 is at 50 kDa.
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