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Western Blot Positive WB detected in: Mouse brain tissue, Rat brain tissue All lanes: HAX1 antibody at 1.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32, 33, 29, 15, 27, 22 kDa Observed band size: 32 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Rat brain tissue All lanes: HAX1 antibody at 1.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32, 33, 29, 15, 27, 22 kDa Observed band size: 32 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Rat brain tissue All lanes: HAX1 antibody at 1.5microg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 32, 33, 29, 15, 27, 22 kDa Observed band size: 32 kDa

HAX1 Antibody

CSB-PA03515A0RB
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHAX1
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Overview

  • Supplier
    Cusabio
  • Product Name
    HAX1 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10456
  • Target name
    HAX1
  • Target description
    HCLS1 associated protein X-1
  • Target synonyms
    HCLSBP1, HS1BP1, SCN3, HCLS1-associated protein X-1, HAX-1, HCLS1 (and PKD2) associated protein, HS1 binding protein, HS1-associating protein X-1, HS1-binding protein 1, HSP1BP-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO00165
  • Protein Name
    HCLS1-associated protein X-1
  • Scientific Description
    Recruits the Arp2/3 complex to the cell cortex and regulates reorganization of the cortical actin cytoskeleton via its interaction with KCNC3 and the Arp2/3 complex (PubMed:26997484). Slows down the rate of inactivation of KCNC3 channels (PubMed:26997484). Promotes GNA13-mediated cell migration. Involved in the clathrin-mediated endocytosis pathway. May be involved in internalization of ABC transporters such as ABCB11. May inhibit CASP9 and CASP3. Promotes cell survival. May regulate intracellular calcium pools.
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of HAX1 using anti-HAX1 antibody (A01495-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human MOLT-4 whole cell lysates, Lane 2: human Raji whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human Hela whole cell lysates, Lane 5: human THP-1 whole cell lysates, Lane 6: human MCF-7 whole cell lysates, Lane 7: human Jurkat whole cell lysates, Lane 8: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HAX1 antigen affinity purified polyclonal antibody (Catalog # A01495-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HAX1 at approximately 36 kDa. The expected band size for HAX1 is at 32 kDa.
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