![ICC/IF analysis of various samples using GTX00702 HB EGF antibody [4G10]. Vero-H : Vero cells overexpressing human HB-EGF protein Vero-mH : Vero cells overexpressing mouse HB-EGF protein Fixation : 4% PFA ICC/IF analysis of various samples using GTX00702 HB EGF antibody [4G10]. Vero-H : Vero cells overexpressing human HB-EGF protein Vero-mH : Vero cells overexpressing mouse HB-EGF protein Fixation : 4% PFA](https://www.genetex.com/upload/website/prouct_img/normal/GTX00702/GTX00702_20191104_ICC-IF_w_23053121_434.webp)
ICC/IF analysis of various samples using GTX00702 HB EGF antibody [4G10]. Vero-H : Vero cells overexpressing human HB-EGF protein Vero-mH : Vero cells overexpressing mouse HB-EGF protein Fixation : 4% PFA
HB EGF antibody [4G10]
GTX00702
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
Product group Antibodies
TargetHBEGF
Overview
- SupplierGeneTex
- Product NameHB EGF antibody [4G10] - Overexpression Validated
- Delivery Days Customer9
- Application Supplier NoteWB: 0.2-1 microg /ml. ICC/IF: 5-10 microg/ml. IP: 2 microg/ml. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, Neutralisation/Blocking
- CertificationResearch Use Only
- ClonalityMonoclonal
- Clone ID4G10
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID1839
- Target nameHBEGF
- Target descriptionheparin binding EGF like growth factor
- Target synonymsdiphtheria toxin receptor (heparin-binding EGF-like growth factor); diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor); DTR; DTS; DTSF; HEGFL; heparin-binding epidermal growth factor; proheparin-binding EGF-like growth factor
- HostMouse
- IsotypeIgG1
- Protein IDQ99075
- Protein NameProheparin-binding EGF-like growth factor
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
References
- Anti-human HB-EGF monoclonal antibodies inhibiting ectodomain shedding of HB-EGF and diphtheria toxin binding. Hamaoka M et al., 2010 Jul, J BiochemRead more
![IP analysis of various samples using GTX00702 HB EGF antibody [4G10]. Lane 1 : Vero whole cell lysate Lane 2 : Vero cells overexpressing human HB-EGF protein Lane 3 : Vero cells overexpressing mouse HB-EGF protein IP analysis of various samples using GTX00702 HB EGF antibody [4G10]. Lane 1 : Vero whole cell lysate Lane 2 : Vero cells overexpressing human HB-EGF protein Lane 3 : Vero cells overexpressing mouse HB-EGF protein](https://www.genetex.com/upload/website/prouct_img/normal/GTX00702/GTX00702_20191104_IP_w_23053121_698.webp)
![WB analysis of various samples using GTX00702 HB EGF antibody [4G10]. HB-EGF is glycosylated at multiple sites and processed, which reflects multiple-forms in WB. Lane 1 : Vero whole cell lysate Lane 2 : Vero cells overexpressing human HB-EGF protein Lane 3 : Vero cells overexpressing mouse HB-EGF protein WB analysis of various samples using GTX00702 HB EGF antibody [4G10]. HB-EGF is glycosylated at multiple sites and processed, which reflects multiple-forms in WB. Lane 1 : Vero whole cell lysate Lane 2 : Vero cells overexpressing human HB-EGF protein Lane 3 : Vero cells overexpressing mouse HB-EGF protein](https://www.genetex.com/upload/website/prouct_img/normal/GTX00702/GTX00702_20191104_WB_w_23053121_245.webp)
![WB analysis of wide-type (WT) and HB EGF knockout (KO) 293T cells using GTX02860 HB EGF antibody [GT1263]. Dilution : 1:1000 Loading : 25microg](https://www.genetex.com/upload/website/prouct_img/normal/GTX02860/CutImage_A11657_KO-WB_01_(1069620)_w_23053123_882.webp)




