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Western Blot analysis of COS7 cells using HCCS Polyclonal Antibody
Western Blot analysis of COS7 cells using HCCS Polyclonal Antibody
Western Blot analysis of COS7 cells using HCCS Polyclonal Antibody

HCCS Antibody

CSB-PA002862
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Monkey, Mouse
TargetHCCS
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Overview

  • Supplier
    Cusabio
  • Product Name
    HCCS Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3052
  • Target name
    HCCS
  • Target description
    holocytochrome c synthase
  • Target synonyms
    CCHL; cytochrome c heme-lyase; cytochrome c-type heme lyase; holocytochrome c-type synthase; holocytochrome-c synthetase; LSDMCA1; MCOPS7; microphthalamia with linear skin defects; microphthalmia with linear skin defects; MLS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53701
  • Protein Name
    Cytochrome c-type heme lyase
  • Reactivity
    Human, Monkey, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HCCS using anti-HCCS antibody (A00924-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCCS antigen affinity purified polyclonal antibody (Catalog # A00924-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCCS at approximately 31 kDa. The expected band size for HCCS is at 31 kDa.
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