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WB analysis of various sample lysates using GTX33236 HCCS antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33236 HCCS antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX33236 HCCS antibody. Dilution : 1:1000 Loading : 25microg per lane

HCCS antibody

GTX33236
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetHCCS
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Overview

  • Supplier
    GeneTex
  • Product Name
    HCCS antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:50 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3052
  • Target name
    HCCS
  • Target description
    holocytochrome c synthase
  • Target synonyms
    CCHL; cytochrome c heme-lyase; cytochrome c-type heme lyase; holocytochrome c-type synthase; holocytochrome-c synthetase; LSDMCA1; MCOPS7; microphthalamia with linear skin defects; microphthalmia with linear skin defects; MLS
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP53701
  • Protein Name
    Cytochrome c-type heme lyase
  • Scientific Description
    The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jan 2010]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HCCS using anti-HCCS antibody (A00924-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human HEK293 whole cell lysates, Lane 5: rat lung tissue lysates, Lane 6: rat stomach tissue lysates, Lane 7: rat PC-12 whole cell lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse stomach tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCCS antigen affinity purified polyclonal antibody (Catalog # A00924-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCCS at approximately 31 kDa. The expected band size for HCCS is at 31 kDa.
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