More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/HCLS1 antibody [N1N3]
HCLS1 antibody immunoprecipitates HCLS1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of HCLS1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of HCLS1 by 2.5 μg of HCLS1 antibody (GTX114467) 10% SDS-PAGE The immunoprecipitated HCLS1 protein was detected by HCLS1 antibody (GTX114467) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
HCLS1 antibody immunoprecipitates HCLS1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of HCLS1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of HCLS1 by 2.5 μg of HCLS1 antibody (GTX114467) 10% SDS-PAGE The immunoprecipitated HCLS1 protein was detected by HCLS1 antibody (GTX114467) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.
HCLS1 antibody immunoprecipitates HCLS1 protein in IP experiments. IP Sample: 293T whole cell lysate/extract A : 30 μg whole cell lysate/extract of HCLS1 protein expressing 293T cells B : Control with 2.5 μg of pre-immune rabbit IgG C : Immunoprecipitation of HCLS1 by 2.5 μg of HCLS1 antibody (GTX114467) 10% SDS-PAGE The immunoprecipitated HCLS1 protein was detected by HCLS1 antibody (GTX114467) diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) (GTX221666-01) was used as a secondary reagent.

HCLS1 antibody [N1N3]

GTX114467
GeneTex
ApplicationsImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetHCLS1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    HCLS1 antibody [N1N3]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. IP: 1:100-1:500. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.95 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3059
  • Target name
    HCLS1
  • Target description
    hematopoietic cell-specific Lyn substrate 1
  • Target synonyms
    CTTNL, HS1, lckBP1, p75, hematopoietic lineage cell-specific protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP14317
  • Protein Name
    Hematopoietic lineage cell-specific protein
  • Scientific Description
    Substrate of the antigen receptor-coupled tyrosine kinase. Plays a role in antigen receptor signaling for both clonal expansion and deletion in lymphoid cells. May also be involved in the regulation of gene expression.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
RayBiotech
Anti-HCLS1 Antibody144-02165
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetHCLS1
  • SizePrice
Figure 1. Western blot analysis of HCLS1 using anti-HCLS1 antibody (A04313-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCLS1 antigen affinity purified polyclonal antibody (Catalog # A04313-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCLS1 at approximately 75 kDa. The expected band size for HCLS1 is at 54 kDa.
Product group Antibodies
Boster Bio
Anti-HCLS1 Antibody Picoband(r)A04313-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetHCLS1
  • SizePrice
Sample (30 ug of whole cell lysate) A: Jurkat B: HL-60 7.5% SDS PAGE GTX100303 diluted at 1:10000
Product group Antibodies
GeneTex
HCLS1 antibody [C3], C-termGTX100303
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetHCLS1
  • SizePrice
Product group Antibodies
Applied Biological Materials
Anti-HCLS1Y058085
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse
  • SizePrice
Lane 1: mouse heart lysates Lane 2: mouse brain lysates probed with Anti HCLS1/LckBP1 Polyclonal Antibody, Unconjugated (bs-5109R) at 1:200 in 4˚C. Followed by conjugation to secondary antibody (bs-0295G-HRP) at 1:3000 90min in 37˚C. Predicted band 53kD. Observed band size: 53kD.
Product group Antibodies
Bioss
HCLS1 Polyclonal AntibodyBS-5109R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Equine, Human, Mouse, Rabbit, Rat
TargetHCLS1
  • SizePrice
Product group Antibodies
Assay Genie
HCLS1 AntibodyCAB2165
ReactivityHuman
TargetHCLS1
  • SizePrice
Anti-HS1 (Phospho-Tyr397) Antibody
Product group Antibodies
Antibodies.com
Anti-HS1 (Phospho-Tyr397) AntibodyA51297
ApplicationsWestern Blot
ReactivityHuman
  • SizePrice
IHC-P analysis of human esophagus tissue using GTX55654 HCLS1 antibody. Dilution : 1:100
Product group Antibodies
GeneTex
HCLS1 antibodyGTX55654
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
ReactivityHuman, Mouse
TargetHCLS1
  • SizePrice
Product group Antibodies
Cusabio
Phospho-HCLS1 (Y378) AntibodyCSB-PA009133
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHCLS1
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
HCLS1 AntibodyLS-C331948
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetHCLS1
  • SizePrice