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Western blot analysis of extracts of various cell lines using HCLS1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using HCLS1 Polyclonal Antibody at dilution of 1:1000.
Western blot analysis of extracts of various cell lines using HCLS1 Polyclonal Antibody at dilution of 1:1000.

HCLS1 Polyclonal Antibody

E-AB-60652
Elabscience
Product group Antibodies

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Overview

  • Supplier
    Elabscience
  • Product Name
    HCLS1 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    HCLS1 (Hematopoietic Cell-Specific Lyn Substrate 1) is a Protein Coding gene. Diseases associated with HCLS1 include Spherocytosis, Type 1. Among its related pathways are Pathogenic Escherichia coli infection and Immune response Fc epsilon RI pathway. Gene Ontology (GO) annotations related to this gene include protein kinase binding. An important paralog of this gene is CTTN.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HCLS1 using anti-HCLS1 antibody (A04313-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human Daudi whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HCLS1 antigen affinity purified polyclonal antibody (Catalog # A04313-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HCLS1 at approximately 75 kDa. The expected band size for HCLS1 is at 54 kDa.
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