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ProductsBack/HDAC10 Mouse Monoclonal Antibody [Clone ID: OTI4D4]
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (RC218536, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10. Positive lysates LY403142 (100ug) and LC403142 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (RC218536, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10. Positive lysates LY403142 (100ug) and LC403142 (20ug) can be purchased separately from OriGene.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (RC218536, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10. Positive lysates LY403142 (100ug) and LC403142 (20ug) can be purchased separately from OriGene.

HDAC10 Mouse Monoclonal Antibody [Clone ID: OTI4D4]

CF500752
OriGene
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot
Product group Antibodies
ReactivityHuman
TargetHDAC10
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    HDAC10 Mouse Monoclonal Antibody [Clone ID: OTI4D4]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4D4
  • Gene ID83933
  • Target name
    HDAC10
  • Target description
    histone deacetylase 10
  • Target synonyms
    HD10, polyamine deacetylase HDAC10
  • Host
    Mouse
  • Isotype
    IgG2a
  • Protein IDQ969S8
  • Protein Name
    Polyamine deacetylase HDAC10
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HDAC10 mouse monoclonal antibody, clone OTI4D4 (formerly 4D4)
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of HDAC10 using anti-HDAC10 antibody (A05215-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human MDA-MB-453 whole cell lysates, Lane 2: human SW620 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC10 antigen affinity purified polyclonal antibody (Catalog # A05215-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC10 at approximately 71KD. The expected band size for HDAC10 is at 71KD.
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