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Western Blot analysis of HepG2 cells using HDAC2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using HDAC2 Polyclonal Antibody
Western Blot analysis of HepG2 cells using HDAC2 Polyclonal Antibody

HDAC2 Antibody

CSB-PA002879
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetHDAC2
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Overview

  • Supplier
    Cusabio
  • Product Name
    HDAC2 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3066
  • Target name
    HDAC2
  • Target description
    histone deacetylase 2
  • Target synonyms
    HD2; histone deacetylase 2; KDAC2; RPD3; transcriptional regulator homolog RPD3; YAF1; YY1-associated factor 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ92769
  • Protein Name
    Histone deacetylase 2
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDAC2 using anti-HDAC2 antibody (A00325-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human THP-1 whole cell lysates, Lane 3: human HL-60 whole cell lysates, Lane 4: human Caco-2 whole cell lysates, Lane 5: human HepG2 whole cell lysates, Lane 6: rat brain tissue lysates, Lane 7: rat lung tissue lysates, Lane 8: rat pancreas tissue lysates, Lane 9: mouse brain tissue lysates, Lane 10: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC2 antigen affinity purified polyclonal antibody (Catalog # A00325-3) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC2 at approximately 60 kDa. The expected band size for HDAC2 is at 60 kDa.
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