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Western Blot analysis of hela cells using HDAC3 Polyclonal Antibody
Western Blot analysis of hela cells using HDAC3 Polyclonal Antibody
Western Blot analysis of hela cells using HDAC3 Polyclonal Antibody

HDAC3 Antibody

CSB-PA002880
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetHDAC3
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Overview

  • Supplier
    Cusabio
  • Product Name
    HDAC3 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID8841
  • Target name
    HDAC3
  • Target description
    histone deacetylase 3
  • Target synonyms
    HD3, KDAC3, RPD3, RPD3-2, histone deacetylase 3, SMAP45, protein deacetylase HDAC3, protein deacylase HDAC3
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDO15379
  • Protein Name
    Histone deacetylase 3
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HDAC3 using anti-HDAC3 antibody (A00839). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human MOLT-4 whole cell lysates, Lane 5: human MCF-7 whole cell lysates, Lane 6: human K562 whole cell lysates, Lane 7: human CACO-2 whole cell lysates, Lane 8: rat thymus tissue lysates, Lane 9: rat C6 whole cell lysates, Lane 10: mouse ANA-1 whole cell lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody (Catalog # A00839) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC3 at approximately 49 kDa. The expected band size for HDAC3 is at 49 kDa.
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