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ProductsBack/HDAC3 antibody [GT9007]
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60366 HDAC3 antibody [GT9007]. A titration of the antibody consisting of 1, 2, 5, and 10 microg per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure 4 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60366 HDAC3 antibody [GT9007]. A titration of the antibody consisting of 1, 2, 5, and 10 microg per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure 4 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).
ChIP analysis of sheared chromatin from 10? HeLa cells using GTX60366 HDAC3 antibody [GT9007]. A titration of the antibody consisting of 1, 2, 5, and 10 microg per ChIP experiment was analysed. IgG (5 microg/IP) was used as negative IP control. QPCR was performed with primers for the promoters of the active genes c-fos and GAPDH, and for the coding region of p21, a known target gene of HDAC3. Figure 4 shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).

HDAC3 antibody [GT9007]

GTX60366
GeneTex
ApplicationsImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
ReactivityHuman
TargetHDAC3
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Overview

  • Supplier
    GeneTex
  • Product Name
    HDAC3 antibody [GT9007]
  • Delivery Days Customer
    9
  • Application Supplier Note
    ICC/IF: 1:500. ChIP assay: 1-5 microg. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ChIP Chromatin ImmunoPrecipitation, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT9007
  • Concentration
    2 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID8841
  • Target name
    HDAC3
  • Target description
    histone deacetylase 3
  • Target synonyms
    HD3; histone deacetylase 3; KDAC3; RPD3; RPD3-2; SMAP45
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDO15379
  • Protein Name
    Histone deacetylase 3
  • Scientific Description
    Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203