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ProductsBack/HDAC4 Mouse Monoclonal Antibody [Clone ID: 7B2]
Western blot analysis using HDAC4 mouse mAb against Hela (1), Jurkat (2) cell lysate.
Western blot analysis using HDAC4 mouse mAb against Hela (1), Jurkat (2) cell lysate.
Western blot analysis using HDAC4 mouse mAb against Hela (1), Jurkat (2) cell lysate.

HDAC4 Mouse Monoclonal Antibody [Clone ID: 7B2]

AM06461SU-N
OriGene
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetHDAC4
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    HDAC4 Mouse Monoclonal Antibody [Clone ID: 7B2]
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7B2
  • Gene ID9759
  • Target name
    HDAC4
  • Target description
    histone deacetylase 4
  • Target synonyms
    AHO3, BDMR, HA6116, HD4, HDAC-4, HDAC-A, HDACA, NEDCHF, NEDCHID, histone deacetylase 4, histone deacetylase A
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP56524
  • Protein Name
    Histone deacetylase 4
  • Scientific Description
    HDAC4 mouse monoclonal antibody, clone 7B2, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDAC4 using anti-HDAC4 antibody (A00971-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysates, Lane 2: human HELA whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HEPG2 whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: mouse testis tissue lysates, Lane 8: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC4 antigen affinity purified polyclonal antibody (Catalog # A00971-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC4 at approximately 140KD. The expected band size for HDAC4 is at 119KD.
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