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Western Blot analysis of K562 cells using HDAC5 Polyclonal Antibody
Western Blot analysis of K562 cells using HDAC5 Polyclonal Antibody
Western Blot analysis of K562 cells using HDAC5 Polyclonal Antibody

HDAC5 Antibody

CSB-PA002888
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetHDAC5
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Overview

  • Supplier
    Cusabio
  • Product Name
    HDAC5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10014
  • Target name
    HDAC5
  • Target description
    histone deacetylase 5
  • Target synonyms
    antigen NY-CO-9; HD5; histone deacetylase 5; NY-CO-9
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9UQL6
  • Protein Name
    Histone deacetylase 5
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HDAC5 using anti-HDAC5 antibody (A01230-5). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: human PC-3 whole cell lysates, Lane 6: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC5 antigen affinity purified polyclonal antibody (Catalog # A01230-5) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC5 at approximately 150KD. The expected band size for HDAC5 is at 122KD.
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