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ProductsBack/HDAC6 Mouse Monoclonal Antibody [Clone ID: OTI4C5]
Simple Western™ analysis of endogenous protein HDAC6 from HELA lysates (0.2 mg/mL) using HDAC6 Mouse Monoclonal Antibody #TA502030. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein HDAC6 from HELA lysates (0.2 mg/mL) using HDAC6 Mouse Monoclonal Antibody #TA502030. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.
Simple Western™ analysis of endogenous protein HDAC6 from HELA lysates (0.2 mg/mL) using HDAC6 Mouse Monoclonal Antibody #TA502030. The virtual lane view (left) shows the target (as indicated) at 1:50 dilution of primary antibody. The corresponding electropherogram view (right) plots chemiluminescence by molecular weight along the capillary at a 1:50 dilution of primary antibody. This experiment was performed under reducing conditions on the Jess™ Simple Western instrument from ProteinSimple, a Bio-Techne brand, using the 12–230 kDa Separation Module.

HDAC6 Mouse Monoclonal Antibody [Clone ID: OTI4C5]

CF502030
OriGene
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityCanine, Human, Monkey, Rat
TargetHDAC6
Price on request
100 ug
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Overview

  • Supplier
    OriGene
  • Product Name
    HDAC6 Mouse Monoclonal Antibody [Clone ID: OTI4C5]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OTI4C5
  • Gene ID10013
  • Target name
    HDAC6
  • Target description
    histone deacetylase 6
  • Target synonyms
    CPBHM, HD6, JM21, KDAC6, PPP1R90, protein deacetylase HDAC6, E3 ubiquitin-protein ligase HDAC6, alpha-tubulin deacetylase HDAC6, protein phosphatase 1, regulatory subunit 90, tubulin-lysine deacetylase HDAC6
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ9UBN7
  • Protein Name
    Protein deacetylase HDAC6
  • Scientific Description
    Carrier-free (BSA/glycerol-free) HDAC6 mouse monoclonal antibody, clone OTI4C5 (formerly 4C5)
  • Reactivity
    Canine, Human, Monkey, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDAC6 using anti-HDAC6 antibody (PB9628). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC6 antigen affinity purified polyclonal antibody (Catalog # PB9628) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC6 at approximately 160 kDa. The expected band size for HDAC6 is at 131 kDa.
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