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Immunohistochemistry of paraffin-embedded Human breast cancer tissue using HDAC6 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human breast cancer tissue using HDAC6 Polyclonal Antibody at dilution 1:50
Immunohistochemistry of paraffin-embedded Human breast cancer tissue using HDAC6 Polyclonal Antibody at dilution 1:50

HDAC6 Polyclonal Antibody

E-AB-14126
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    HDAC6 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Applications Supplier
    ELISA IHC
  • Certification
    Research Use Only
  • Concentration
    0.8mg/ml
  • Scientific Description
    Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HDAC6 using anti-HDAC6 antibody (PB9628). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human K562 whole cell lysates, Lane 2: human HepG2 whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: rat brain tissue lysates, Lane 5: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC6 antigen affinity purified polyclonal antibody (Catalog # PB9628) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HDAC6 at approximately 160 kDa. The expected band size for HDAC6 is at 131 kDa.
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