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HE4 antibody (FITC)

ORB102716
Biorbyt
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
Product group Antibodies
TargetWFDC2
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Overview

  • Supplier
    Biorbyt
  • Product Name
    HE4 antibody (FITC)
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Applications Supplier
    IF/ICC:1:50-200 (based on 0.5 mg/ml) ICC, IF
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    FITC
  • Gene ID10406
  • Target name
    WFDC2
  • Target description
    WAP four-disulfide core domain 2
  • Target synonyms
    dJ461P17.6; EDDM4; epididymal protein 4; epididymal secretory protein E4; epididymis secretory sperm binding protein; epididymis-specific, whey-acidic protein type, four-disulfide core; HE4; human epididymis protein 4; major epididymis-specific protein E4; putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; WAP four-disulfide core domain protein 2; WAP5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ14508
  • Protein Name
    WAP four-disulfide core domain protein 2
  • Scientific Description
    HE4 antibody (FITC)
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HE4 using anti-HE4 antibody (A02685-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MDA-MB-231 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody (Catalog # A02685-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HE4 at approximately 15KD. The expected band size for HE4 is at 13KD.
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