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HE4 Antibody (YA889, Rabbit)

HY-P81043
MedChem Express
TargetWFDC2
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    HE4 Antibody (YA889, Rabbit)
  • Delivery Days Customer
    14
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID10406
  • Target name
    WFDC2
  • Target description
    WAP four-disulfide core domain 2
  • Target synonyms
    BENP, EDDM4, HE4, WAP5, dJ461P17.6, WAP four-disulfide core domain protein 2, WAP domain containing protein HE4-V4, epididymal protein 4, epididymal secretory protein E4, epididymis secretory sperm binding protein, epididymis-specific, whey-acidic protein type, four-disulfide core, human epididymis protein 4, major epididymis-specific protein E4, putative protease inhibitor WAP5
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    HE4 Antibody (YA889) is an unconjugated, approximately 13-19 kDa, rabbit-derived, anti-HE4 (YA889) monoclonal antibody. HE4 Antibody (YA889) can be used for: WB, IF-Cell, IF-Tissue, IHC-P, IP, FC expriments in human, mouse background without labeling.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of HE4 using anti-HE4 antibody (A02685-4). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MDA-MB-231 whole cell lysates, Lane 3: human MDA-MB-453 whole cell lysates, Lane 4: rat thymus tissue lysates, Lane 5: mouse testis tissue lysates, Lane 6: mouse thymus tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HE4 antigen affinity purified polyclonal antibody (Catalog # A02685-4) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HE4 at approximately 15KD. The expected band size for HE4 is at 13KD.
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