Bio-Connect

Heavy chain cardiac Myosin antibody [3-48]

GTX20015
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
Product group Antibodies
TargetMYH6
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request

Overview

  • Supplier
    GeneTex
  • Product Name
    Heavy chain cardiac Myosin antibody [3-48]
  • Delivery Days Customer
    9
  • Application Supplier Note
    For ELISA: Use at an assay dependant dilution. For IF: Use at an assay dependant dilution. For IHC-Fr: Use at an assay dependant dilution. For WB: Use at an assay dependant dilution. Predicted molecular weight: 223 kDa. Optimal dilutions/concentrations should be determined by the researcher.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3-48
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4624
  • Target name
    MYH6
  • Target description
    myosin heavy chain 6
  • Target synonyms
    alpha-MHC; ASD3; CMD1EE; CMH14; MYHC; MYHCA; myHC-alpha; myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1); myosin-6; SSS3
  • Host
    Mouse
  • Isotype
    IgG1
  • Scientific Description
    3-48 antibody cross-reacts with heavy chains of human myosin isolated from: slow skeletal muscle, fast skeletal muscle, eye muscle, and streptococcal M5 peptide. It does not react with human platelet, smooth muscle myosin heavy chains, myosin light chains, myosin fragments circulating in blood of patients with myocardial infarction, nor does it react with any other human tissue antigens or blood cells.
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • CircCLTH promotes skeletal muscle development and regeneration. Chen M et al., 2022 Dec, Epigenetics
    Read more
  • CircRIMKLB promotes myoblast proliferation and inhibits differentiation by sponging miR-29c to release KCNJ12.
    Read more
  • The circular RNA circCPE regulates myoblast development by sponging miR-138. Ru W et al., 2021 Sep 8, J Anim Sci Biotechnol
    Read more
  • Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro. Flavie Ouali BE et al., 2021 Jul, Biochem Biophys Rep
    Read more
  • CircINSR Regulates Fetal Bovine Muscle and Fat Development. Shen X et al., 2020, Front Cell Dev Biol
    Read more
  • Circular RNA circMYL1 Inhibit Proliferation and Promote Differentiation of Myoblasts by Sponging miR-2400. Elnour IE et al., 2021 Jan 16, Cells
    Read more
  • Familial Recurrent Myocarditis Triggered by Exercise in Patients With a Truncating Variant of the Desmoplakin Gene. Poller W et al., 2020 May 18, J Am Heart Assoc
    Read more
  • Bta-miR-885 promotes proliferation and inhibits differentiation of myoblasts by targeting MyoD1. Elsaeid Elnour I et al., 2020 Oct, J Cell Physiol
    Read more
  • Circular RNA TTN Acts As a miR-432 Sponge to Facilitate Proliferation and Differentiation of Myoblasts via the IGF2/PI3K/AKT Signaling Pathway. Wang X et al., 2019 Dec 6, Mol Ther Nucleic Acids
    Read more
  • Bioelectronic Recordings of Cardiomyocytes with Accumulation Mode Electrolyte Gated Organic Field Effect Transistors. Kyndiah A et al., 2020 Feb 15, Biosens Bioelectron
    Read more

Related products

IHC image of CSB-PA015299HA01HU diluted at 1:100 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Product group Antibodies
Cusabio
MYH6 AntibodyCSB-PA015299HA01HU
ApplicationsELISA, ImmunoHistoChemistry
TargetMYH6
  • SizePrice
Product group Antibodies
Biomatik
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
TargetMYH6
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetMYH6
  • SizePrice
Product group Antibodies
GeneTex
ApplicationsWestern Blot
TargetMYH6
  • SizePrice
Product group Antibodies
RayBiotech
ApplicationsWestern Blot
TargetMYH6
  • SizePrice
Immunohistochemistry analysis in human heart muscle and liver tissues using AMAb90947 antibody. Corresponding MYH6 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
ApplicationsWestern Blot, ImmunoHistoChemistry
TargetMYH6
  • SizePrice
Figure 1. Western blot analysis of MYH6 using anti-MYH6 antibody (A02808-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH6 antigen affinity purified polyclonal antibody (Catalog # A02808-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYH6 at approximately 224 kDa. The expected band size for MYH6 is at 224 kDa.
Product group Antibodies
Boster Bio
Anti-MYH6 Antibody Picoband(r)A02808-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
TargetMYH6
  • SizePrice
Human skeletal muscle lysates probed with Myosin heavy chain (8C1) Monoclonal Antibody (bsm-52867R) at 1:1000 overnight at 4°C followed by a conjugated secondary antibody for 60 minutes at 37°C.
Product group Antibodies
Bioss Antibodies
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetMYH6
  • SizePrice
Product group Antibodies
MedChem Express
TargetMYH6
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
ApplicationsWestern Blot
TargetMYH6
  • SizePrice