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Myosin heavy chain Antibody (YA3200, Rabbit)

HY-P83455
MedChem Express
TargetMYH6
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    Myosin heavy chain Antibody (YA3200, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID4624
  • Target name
    MYH6
  • Target description
    myosin heavy chain 6
  • Target synonyms
    ASD3, CMD1EE, CMH14, MYHC, MYHCA, SSS3, alpha-MHC, myosin-6, myHC-alpha, myosin, heavy polypeptide 6, cardiac muscle, alpha (cardiomyopathy, hypertrophic 1)
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    Myosin heavy chain Antibody (YA3200) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA3200), targeting MYH6, with a predicted molecular weight of 224 kDa. Myosin heavy chain Antibody (YA3200) can be used for WB, IHC-P experiment in human, mouse, rat background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of MYH6 using anti-MYH6 antibody (A02808-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MYH6 antigen affinity purified polyclonal antibody (Catalog # A02808-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MYH6 at approximately 224 kDa. The expected band size for MYH6 is at 224 kDa.
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