More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/Heme Oxygenase 1 antibody [GT1334]
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT1334] (GTX633676) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT1334] (GTX633676) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Heme Oxygenase 1 antibody [GT1334] (GTX633676) diluted at 1:5000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Heme Oxygenase 1 antibody [GT1334]

GTX633676
GeneTex
ApplicationsWestern Blot
Product group Antibodies
ReactivityHuman
TargetHMOX1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    Heme Oxygenase 1 antibody [GT1334]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    GT1334
  • Concentration
    2.09 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3162
  • Target name
    HMOX1
  • Target description
    heme oxygenase 1
  • Target synonyms
    HMOX1D, HO-1, HSP32, bK286B10, heme oxygenase 1, heat shock protein, 32-kD, heme oxygenase (decycling) 1
  • Host
    Mouse
  • Isotype
    IgG2b
  • Protein IDP09601
  • Protein Name
    Heme oxygenase 1
  • Scientific Description
    Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of Heme Oxygenase 1/HMOX1 using anti-Heme Oxygenase 1/HMOX1 antibody (A00253-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme Oxygenase 1/HMOX1 antigen affinity purified polyclonal antibody (Catalog # A00253-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme Oxygenase 1/HMOX1 at approximately 33 kDa. The expected band size for Heme Oxygenase 1/HMOX1 is at 33 kDa.
Product group Antibodies
Boster Bio
Anti-Heme Oxygenase 1/HMOX1 Antibody Picoband(r)A00253-2-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetHMOX1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-HMOX1 Antibody144-11102
ApplicationsImmunoFluorescence, Western Blot
ReactivityHuman, Mouse, Rat
TargetHMOX1
  • SizePrice
Immunohistochemistry analysis in human spleen and skeletal muscle tissues using AMAb91719 antibody. Corresponding HMOX1 RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-HMOX1 AntibodyAMAB91719
ApplicationsImmunoHistoChemistry
ReactivityHuman
TargetHMOX1
  • SizePrice
Product group Antibodies
Biosynth
Heme Oxygenase 1 antibody10R-H112A
  • SizePrice
Product group Antibodies
Biomatik
Heme Oxygenase 1 (HO1) Polyclonal AntibodyCAU27603
ApplicationsImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityMouse, Porcine
TargetHMOX1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Product group Antibodies
References
Bioss
Heme oxygenase 1 Polyclonal AntibodyBS-2075R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetHMOX1
  • SizePrice
Immunocytochemistry analysis of human liver cancer using CSB-PA010583LA01HU at dilution of 1:100
Product group Antibodies
Cusabio
HMOX1 AntibodyCSB-PA010583LA01HU
ApplicationsImmunoFluorescence, ELISA, ImmunoHistoChemistry
ReactivityHuman
TargetHMOX1
  • SizePrice
Product group Antibodies
Assay Genie
AK1 AntibodyCAB13460
ReactivityHuman
TargetHMOX1
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-Heme Oxygenase 1 (aa242-256) AntibodyEB11744
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetHMOX1
  • SizePrice
Product group Antibodies
MedChem Express
Heme Oxygenase 1 Antibody (YA389, Rabbit)HY-P80499
TargetHMOX1
  • SizePrice