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Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

Heme oxygenase 1 Polyclonal Antibody

BS-2075R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
TargetHMOX1
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Overview

  • Supplier
    Bioss
  • Product Name
    Heme oxygenase 1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3162
  • Target name
    HMOX1
  • Target description
    heme oxygenase 1
  • Target synonyms
    HMOX1D, HO-1, HSP32, bK286B10, heme oxygenase 1, heat shock protein, 32-kD, heme oxygenase (decycling) 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09601
  • Protein Name
    Heme oxygenase 1
  • Reactivity
    Bovine, Canine, Chicken, Equine, Guinea Pig, Human, Mouse, Porcine, Rabbit, Rat, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

References

  • Baicalin mitigated Mycoplasma gallisepticum-induced structural damage and attenuated oxidative stress and apoptosis in chicken thymus through the Nrf2/HO-1 defence pathway. Li J et al., 2019 Oct 21, Vet Res
    Read this paper
  • Central administration of tert-butylhydroquinone attenuates hypertension via regulating Nrf2 signaling in the hypothalamic paraventricular nucleus of hypertensive rats. Bai J et al., 2017 Oct 15, Toxicol Appl Pharmacol
    Read this paper
  • Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. Maiga S et al., 2017, PLoS One
    Read this paper
  • TBHQ Alleviated Endoplasmic Reticulum Stress-Apoptosis and Oxidative Stress by PERK-Nrf2 Crosstalk in Methamphetamine-Induced Chronic Pulmonary Toxicity. Wang Y et al., 2017, Oxid Med Cell Longev
    Read this paper
  • Tert-Butylhydroquinone Protects Liver Against Ischemia/Reperfusion Injury in Rats Through Nrf2-Activating Anti-Oxidative Activity. Zeng XP et al., 2017 Mar, Transplant Proc
    Read this paper
  • Fluoxetine protects against methamphetamine-induced lung inflammation by suppressing oxidative stress through the SERT/p38 MAPK/Nrf2 pathway in rats. Wang Y et al., 2017 Feb, Mol Med Rep
    Read this paper
  • Astaxanthin blocks preeclampsia progression by suppressing oxidative stress and inflammation. Xuan RR et al., 2016 Sep, Mol Med Rep
    Read this paper
  • Protective effect of heme oxygenase-1 on Wistar rats with heart failure through the inhibition of inflammation and amelioration of intestinal microcirculation. Zhang L et al., 2015 Jul, J Geriatr Cardiol
    Read this paper
  • Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling. Ni S et al., 2015, Int J Clin Exp Pathol
    Read this paper
  • The cardioprotective effect of sodium tanshinone IIA sulfonate and the optimizing of therapeutic time window in myocardial ischemia/reperfusion injury in rats. Wei B et al., 2014 Aug, Atherosclerosis
    Read this paper

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Figure 1. Western blot analysis of Heme Oxygenase 1/HMOX1 using anti-Heme Oxygenase 1/HMOX1 antibody (A00253-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme Oxygenase 1/HMOX1 antigen affinity purified polyclonal antibody (Catalog # A00253-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme Oxygenase 1/HMOX1 at approximately 33 kDa. The expected band size for Heme Oxygenase 1/HMOX1 is at 33 kDa.
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