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Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining

Heme oxygenase 1 Polyclonal Antibody

BS-2075R
Bioss
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHMOX1
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Overview

  • Supplier
    Bioss
  • Product Name
    Heme oxygenase 1 Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID3162
  • Target name
    HMOX1
  • Target description
    heme oxygenase 1
  • Target synonyms
    HMOX1D, HO-1, HSP32, bK286B10, heme oxygenase 1, heat shock protein, 32-kD, heme oxygenase (decycling) 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09601
  • Protein Name
    Heme oxygenase 1
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Li J, Qiao Z, Hu W, et al. Baicalin mitigated Mycoplasma gallisepticum-induced structural damage and attenuated oxidative stress and apoptosis in chicken thymus through the Nrf2/HO-1 defence pathway. Vet Res. 2019,50(1):83. doi: 10.1186/s13567-019-0703-6
    Read this paper
  • Bai J, Yu XJ, Liu KL, et al. Central administration of tert-butylhydroquinone attenuates hypertension via regulating Nrf2 signaling in the hypothalamic paraventricular nucleus of hypertensive rats. Toxicol Appl Pharmacol. 2017,333:100-109. doi: 10.1016/j.taap.2017.08.012
    Read this paper
  • Maïga S, Allain G, Hauet T, et al. Renal auto-transplantation promotes cortical microvascular network remodeling in a preclinical porcine model. PLoS One. 2017,12(7):e0181067. doi: 10.1371/journal.pone.0181067
    Read this paper
  • Wang Y, Gu YH, Liu M, et al. TBHQ Alleviated Endoplasmic Reticulum Stress-Apoptosis and Oxidative Stress by PERK-Nrf2 Crosstalk in Methamphetamine-Induced Chronic Pulmonary Toxicity. Oxid Med Cell Longev. 2017,2017:4310475. doi: 10.1155/2017/4310475
    Read this paper
  • Zeng XP, Li XJ, Zhang QY, et al. Tert-Butylhydroquinone Protects Liver Against Ischemia/Reperfusion Injury in Rats Through Nrf2-Activating Anti-Oxidative Activity. Transplant Proc. 2017,49(2):366-372. doi: 10.1016/j.transproceed.2016.12.008
    Read this paper
  • Wang Y, Gu YH, Liu M, et al. Fluoxetine protects against methamphetamine‑induced lung inflammation by suppressing oxidative stress through the SERT/p38 MAPK/Nrf2 pathway in rats. Mol Med Rep. 2017,15(2):673-680. doi: 10.3892/mmr.2016.6072
    Read this paper
  • Xuan RR, Niu TT, Chen HM. Astaxanthin blocks preeclampsia progression by suppressing oxidative stress and inflammation. Mol Med Rep. 2016,14(3):2697-704. doi: 10.3892/mmr.2016.5569
    Read this paper
  • Zhang L, Gan ZK, Han LN, et al. Protective effect of heme oxygenase-1 on Wistar rats with heart failure through the inhibition of inflammation and amelioration of intestinal microcirculation. J Geriatr Cardiol. 2015,12(4):353-65. doi: 10.11909/j.issn.1671-5411.2015.04.001
    Read this paper
  • Ni S, Wang D, Qiu X, et al. Bone marrow mesenchymal stem cells protect against bleomycin-induced pulmonary fibrosis in rat by activating Nrf2 signaling. Int J Clin Exp Pathol. 2015,8(7):7752-61.
    Read this paper
  • Wei B, Li WW, Ji J, et al. The cardioprotective effect of sodium tanshinone IIA sulfonate and the optimizing of therapeutic time window in myocardial ischemia/reperfusion injury in rats. Atherosclerosis. 2014,235(2):318-27. doi: 10.1016/j.atherosclerosis.2014.05.924
    Read this paper

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Figure 1. Western blot analysis of Heme Oxygenase 1/HMOX1 using anti-Heme Oxygenase 1/HMOX1 antibody (A00253-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme Oxygenase 1/HMOX1 antigen affinity purified polyclonal antibody (Catalog # A00253-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme Oxygenase 1/HMOX1 at approximately 33 kDa. The expected band size for Heme Oxygenase 1/HMOX1 is at 33 kDa.
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