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ProductsBack/Heme Oxygenase 1 antibody
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).
Heme Oxygenase 1 antibody detects Heme Oxygenase 1 protein at endoplasmic reticulum and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Heme Oxygenase 1 stained by Heme Oxygenase 1 antibody (GTX101147) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).

Heme Oxygenase 1 antibody

GTX101147
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Monkey, Mouse, Rat
TargetHMOX1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Heme Oxygenase 1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.54 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3162
  • Target name
    HMOX1
  • Target description
    heme oxygenase 1
  • Target synonyms
    HMOX1D, HO-1, HSP32, bK286B10, heme oxygenase 1, heat shock protein, 32-kD, heme oxygenase (decycling) 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09601
  • Protein Name
    Heme oxygenase 1
  • Scientific Description
    Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]
  • Reactivity
    Human, Monkey, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Heme Oxygenase 1/HMOX1 using anti-Heme Oxygenase 1/HMOX1 antibody (A00253-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme Oxygenase 1/HMOX1 antigen affinity purified polyclonal antibody (Catalog # A00253-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme Oxygenase 1/HMOX1 at approximately 33 kDa. The expected band size for Heme Oxygenase 1/HMOX1 is at 33 kDa.
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Paraformaldehyde-fixed, paraffin embedded rat lung tissue; Antigen retrieval by boiling in sodium citrate buffer(pH6) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 30 minutes; Blocking buffer (normal goat serum) at 37°C for 20min; Antibody incubation with Rabbit Anti-Heme Oxygenase Polyclonal Antibody, Unconjugated (bs-2075) at 1:500 overnight at 4°C, followed by a conjugated secondary and DAB staining
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