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Heme Oxygenase 1 antibody

GTX101147
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetHMOX1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Heme Oxygenase 1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.54 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID3162
  • Target name
    HMOX1
  • Target description
    heme oxygenase 1
  • Target synonyms
    HMOX1D, HO-1, HSP32, bK286B10, heme oxygenase 1, heat shock protein, 32-kD, heme oxygenase (decycling) 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09601
  • Protein Name
    Heme oxygenase 1
  • Scientific Description
    Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Tsai LT, Weng TI, Chang TY, et al. Inhibition of Indoxyl Sulfate-Induced Reactive Oxygen Species-Related Ferroptosis Alleviates Renal Cell Injury In Vitro and Chronic Kidney Disease Progression In Vivo. Antioxidants (Basel). 2023,12(11). doi: 10.3390/antiox12111931
    Read this paper
  • Sulistyowati E, Huang SE, Cheng TL, et al. Vasculoprotective Potential of Baicalein in Angiotensin II-Infused Abdominal Aortic Aneurysms through Inhibiting Inflammation and Oxidative Stress. Int J Mol Sci. 2023,24(21). doi: 10.3390/ijms242116004
    Read this paper
  • Shou JW, Shaw PC. Berberine Reduces Lipid Accumulation in Obesity via Mediating Transcriptional Function of PPARδ. Int J Mol Sci. 2023,24(14). doi: 10.3390/ijms241411600
    Read this paper
  • Consoli V, Burò I, Gulisano M, et al. Evaluation of the Antioxidant and Antiangiogenic Activity of a Pomegranate Extract in BPH-1 Prostate Epithelial Cells. Int J Mol Sci. 2023,24(13). doi: 10.3390/ijms241310719
    Read this paper
  • Kogami M, Abe S, Nakamura H, et al. Fenofibrate attenuates the cytotoxic effect of cisplatin on lung cancer cells by enhancing the antioxidant defense system in vitro. Oncol Lett. 2023,26(1):313. doi: 10.3892/ol.2023.13899
    Read this paper
  • Lee YP, Lin CR, Chen SS, et al. Combination treatment of cordycepin and radiation induces MA-10 mouse Leydig tumor cell death via ROS accumulation and DNA damage. Am J Cancer Res. 2023,13(4):1329-1346.
    Read this paper
  • Zhang W, Fan Z, Wang F, et al. Tubeimoside I Ameliorates Doxorubicin-Induced Cardiotoxicity by Upregulating SIRT3. Oxid Med Cell Longev. 2023,2023:9966355. doi: 10.1155/2023/9966355
    Read this paper
  • Lai W, Li D, Wang Q, et al. Bacterial Magnetosomes Release Iron Ions and Induce Regulation of Iron Homeostasis in Endothelial Cells. Nanomaterials (Basel). 2022,12(22). doi: 10.3390/nano12223995
    Read this paper
  • Pritchard KA Jr, Jing X, Teng M, et al. Role of endoplasmic reticulum stress in impaired neonatal lung growth and bronchopulmonary dysplasia. PLoS One. 2022,17(8):e0269564. doi: 10.1371/journal.pone.0269564
    Read this paper
  • Ciaffaglione V, Consoli V, Intagliata S, et al. Novel Tyrosine Kinase Inhibitors to Target Chronic Myeloid Leukemia. Molecules. 2022,27(10). doi: 10.3390/molecules27103220
    Read this paper

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Figure 1. Western blot analysis of Heme Oxygenase 1/HMOX1 using anti-Heme Oxygenase 1/HMOX1 antibody (A00253-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human A549 whole cell lysates, Lane 3: human 293T whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Heme Oxygenase 1/HMOX1 antigen affinity purified polyclonal antibody (Catalog # A00253-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Heme Oxygenase 1/HMOX1 at approximately 33 kDa. The expected band size for Heme Oxygenase 1/HMOX1 is at 33 kDa.
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