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WB analysis of 293 cell lysate (35ug/lane) using GTX53555 Hemoglobin theta 1 antibody.
WB analysis of 293 cell lysate (35ug/lane) using GTX53555 Hemoglobin theta 1 antibody.
WB analysis of 293 cell lysate (35ug/lane) using GTX53555 Hemoglobin theta 1 antibody.

Hemoglobin theta 1 antibody

GTX53555
GeneTex
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetHBQ1
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Overview

  • Supplier
    GeneTex
  • Product Name
    Hemoglobin theta 1 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:1000. IHC-P: 1:10-1:50. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID3049
  • Target name
    HBQ1
  • Target description
    hemoglobin subunit theta 1
  • Target synonyms
    HBQ; hemoglobin subunit theta-1; hemoglobin theta-1 chain; hemoglobin, theta 1; theta-1-globin
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09105
  • Protein Name
    Hemoglobin subunit theta-1
  • Scientific Description
    Theta-globin mRNA is found in human fetal erythroid tissue but not in adult erythroid or other nonerythroid tissue. The theta-1 gene may be expressed very early in embryonic life, perhaps sometime before 5 weeks. Theta-1 is a member of the human alpha-globin gene cluster that involves five functional genes and two pseudogenes. The order of genes is: 5 - zeta - pseudozeta - mu - pseudoalpha-2 -pseudoalpha-1 - alpha-2 - alpha-1 - theta-1 - 3. Research supports a transcriptionally active role for the gene and a functional role for the peptide in specific cells, possibly those of early erythroid tissue. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HBQ1 using anti-HBQ1 antibody (A14380-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HBQ1 antigen affinity purified polyclonal antibody (Catalog # A14380-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HBQ1 at approximately 16 kDa. The expected band size for HBQ1 is at 16 kDa.
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