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HERPUD1 / HERP Antibody

LS-C749110
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
TargetHERPUD1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    HERPUD1 / HERP Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 26kDa/40kDa/43kDa, while the observed MW by Western blot was 44kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 26kDa/40kDa/43kDa, while the observed MW by Western blot was 44kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9709
  • Target name
    HERPUD1
  • Target description
    homocysteine inducible ER protein with ubiquitin like domain 1
  • Target synonyms
    HERP, HERPUD1-IT1, Mif1, SUP, homocysteine-responsive endoplasmic reticulum-resident ubiquitin-like domain member 1 protein, HERPUD1 intronic transcript 1, MMS-inducible, homocysteine-inducible endoplasmic reticulum stress-inducible ubiquitin-like domain member 1 protein, homocysteine-inducible, endoplasmic reticulum stress-inducible, ubiquitin-like domain member 1, methyl methanesulfonate (MMF)-inducible fragment protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HERP/HERPUD1 using anti-HERP/HERPUD1 antibody (A04908-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human PC-3 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human HepG2 whole cell lysates, Lane 4: human LNCAP whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HERP/HERPUD1 antigen affinity purified polyclonal antibody (Catalog # A04908-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HERP/HERPUD1 at approximately 56 kDa. The expected band size for HERP/HERPUD1 is at 44 kDa.
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