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Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Mouse skin tissue, human fetal liver tissue, Human fetal brain tissue, Primary antibody: CSB-PA041972(HES5 Antibody) at dilution 1/100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Mouse skin tissue, human fetal liver tissue, Human fetal brain tissue, Primary antibody: CSB-PA041972(HES5 Antibody) at dilution 1/100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Mouse skin tissue, human fetal liver tissue, Human fetal brain tissue, Primary antibody: CSB-PA041972(HES5 Antibody) at dilution 1/100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 2 minutes

HES5 Antibody

CSB-PA041972
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
TargetHES5
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Overview

  • Supplier
    Cusabio
  • Product Name
    HES5 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID388585
  • Target name
    HES5
  • Target description
    hes family bHLH transcription factor 5
  • Target synonyms
    bHLHb38; class B basic helix-loop-helix protein 38; hairy and enhancer of split 5; transcription factor HES-5
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ5TA89
  • Protein Name
    Transcription factor HES-5
  • Scientific Description
    This gene encodes a member of a family of basic helix-loop-helix transcriptional repressors. The protein product of this gene, which is activated downstream of the Notch pathway, regulates cell differentiation in multiple tissues. Disruptions in the normal expression of this gene have been associated with developmental diseases and cancer.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of HES5 using anti-HES5 antibody (A05384-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hacat whole cell lysates, Lane 2: human HepG2 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HES5 antigen affinity purified polyclonal antibody (Catalog # A05384-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HES5 at approximately 18 kDa. The expected band size for HES5 is at 18 kDa.
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